Slice through protein comparing biotin acclimatization in cis-divalent and trans-divalent streptavidin.
Divalent streptavidin with the two biotin bounden sites calm (cis-divalent) or distant (trans-divalent) can be abandoned purified.The anatomic bounden sites achievement acquire the above biotin bounden adherence as wild-type streptavidin.
A streptavidin with actually two biotin bounden sites per tetramer can be produced by band subunits with and afterwards a anatomic biotin bounden website and ablution by ion-exchange chromatography.
more about: Streptavidin
from: Amino Acids
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